Patent · US Active

Method for making polypeptides

US8815541B2 · kind B2 · utility

0Cited by

5References

16Claims

0Family size

Assignee

Novo Nordisk A/S · DK

Inventors

Asser Sloth Andersen · Copenhagen, DK
Inger Lautrup-Larsen · Avenue Madeleine, DK
Per Noergaard · Rødby, DK

Key dates

Filing date	Nov 25, 2010
Grant date	Aug 26, 2014
Priority date	—
Expiry date	Nov 25, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P21/02
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention provides an improved method for producing polypeptides with a C-terminal glycine in a yeast transformant being characterized in having a non functional KEX1 gene. The method is in particular well suited to produce polypeptides with an aromatic amino acid residue attached to the C-terminal glycine. The yeast strain may have further non-functional protease genes selected from PEP4, YPS1, MKCI, YPS3, YPS5, YPS6, YPS7, PRB1, STE13 and KEX2.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.