Methods of producing antigen-specific B cell populations in the presence of CD40:CD40L, BAFF:BAFF receptor and Fas:FasL stimulation
US8815543B2 · kind B2 · utility
2Cited by
1References
6Claims
0Family size
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Key dates
| Filing date | Oct 25, 2010 |
| Grant date | Aug 26, 2014 |
| Priority date | — |
| Expiry date | Oct 25, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for producing an antigen-specific B cell population comprising IgG-positive B cells specific to a specific antigen, the method comprising: culturing IgG-positive B cells together with the specific antigen in the presence of IL-21, while conferring stimulation to the IgG-positive B cells via CD40, a BAFF receptor and Fas; and screening for antigen-specific B cells specific to the specific antigen to obtain an antigen-specific B cell population comprising the IgG-positive B cells specific to the specific antigen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.