Serum-free mammalian cell culture medium, and uses thereof
US8815573B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 20, 2013 |
| Grant date | Aug 26, 2014 |
| Priority date | — |
| Expiry date | Mar 20, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/10051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells. The invention also provides kits for use in the cultivation of a mammalian epithelial cell that comprise one or more containers, wherein a first container contains the culture medium of the invention. These kits may further comprise one or more additional containers containing one or more supplements.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.