Patent · US Active

Chip-based sequencing nucleic acids

US8815576B2 · kind B2 · utility

1Cited by
4References
20Claims
0Family size

Assignee

Inventor

Key dates

Filing dateDec 27, 2007
Grant dateAug 26, 2014
Priority date—
Expiry dateMay 18, 2032

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01L2400/0487
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.