Cells producing glycoproteins having altered glycosylation patterns and method and use thereof

US8815580B2 · kind B2 · utility

Assignees

• VIB VZW · BE
• UNIVERSITEIT GENT · BE

Inventors

• Nico Callewaert · Nevele, BE
• Karen Jacqueline Marcel De Pourcq · Evergem, BE
• Steven Christian Jozef Geysens · Kruisem, BE
• Leander Meuris · Evergem, BE

Key dates

• Filing date: Aug 10, 2009
• Grant date: Aug 26, 2014
• Expiry date: Feb 6, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present application relates to the field of glyco-engineering, more specifically to eukaryotic cells wherein both an endoglucosaminidase and a glycoprotein are present. These cells can be used to deglycosylate or partly deglycosylate the (exogenous) glycoprotein, in particular without the need for adding an extra enzyme. Methods are also provided for the application of these cells in protein production. According to one specific aspect, the eukaryotic cells and methods are glyco-engineered yeast cells in which additionally at least one exogenous enzyme needed for complex glycosylation is present, e.g. allowing easier separation of differentially glycosylated glycoproteins.