Homologous recombination-based DNA cloning methods and compositions
US8815600B2 · kind B2 · utility
44Cited by
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13Claims
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Key dates
| Filing date | Mar 5, 2010 |
| Grant date | Aug 26, 2014 |
| Priority date | — |
| Expiry date | Jul 27, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.