Use of DNAzymes for analysis of an RNA sample
US8815782B2 · kind B2 · utility
20Cited by
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17Claims
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Key dates
| Filing date | Nov 1, 2012 |
| Grant date | Aug 26, 2014 |
| Priority date | — |
| Expiry date | Nov 7, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein is method comprising: contacting an initial RNA sample containing a population of different RNA molecules with a divalent cation and a set of DNAzymes that are designed to cleave multiple target RNAs in the initial sample, thereby producing a product RNA sample that comprises: a) uncleaved RNA molecules and b) cleaved RNA fragments that contain a 2′,3′-cyclic-phosphate and a 5′ hydroxyl as the result of DNAzyme cleavage.
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