Nucleic acid purification method

US8816063B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Jan Petzel · Tübingen, DE
• Holger Wedler · Hilden, DE
• Roland Fabis · Leverkusen, DE

Key dates

• Filing date: May 11, 2010
• Grant date: Aug 26, 2014
• Expiry date: Jul 17, 2030

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T428/2991
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for purifying a defined amount of nucleic acids from a nucleic acid-containing sample, which has at least the following steps: (a.) contacting the nucleic acid-containing sample with a defined amount of a nucleic acid binding phase with the following features: (i) the nucleic acid binding phase has nucleic acid binding ligands that have at least one protonatable group; (ii) the nucleic acid binding ligands are bound to a carrier; (iii) the nucleic acid binding phase has a surface with a low charge density, wherein the amount of nucleic acids in the sample exceeds the binding capacity of the amount of nucleic acid binding phase used; (b.) binding of the nucleic acids to the nucleic acid binding phase at a pH (binding pH) that is below the pKs value of at least one of the protonatable groups; (c.) elution of the nucleic acids at a pH that is above the binding pH, wherein a defined amount of nucleic acids is obtained. Furthermore, corresponding kits and nucleic acid binding phases, which can be used for the purification of nucleic acids, are disclosed.