Recombinant DNA constructs employing site-specific recombination
US8816153B2 · kind B2 · utility
9Cited by
2References
15Claims
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Key dates
| Filing date | Aug 27, 2010 |
| Grant date | Aug 26, 2014 |
| Priority date | — |
| Expiry date | Aug 27, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8243
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.