Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases
US8822146B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 1, 2010 |
| Grant date | Sep 2, 2014 |
| Priority date | — |
| Expiry date | Jun 23, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/91011
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a use of non-cofactor compounds, represented by formulas (I) or (II) wherein R and Z are independently selected from H, D, C1-C12-alkyl, preferably C1-C4-alkyl, alkenyl, alkinyl, phenyl or -LX, wherein X represents a functional group or a reporter group attached via a linker group L, and QH is selected from —SH, —SeH, —NHNH2 or —ONH2, for a targeted modification or derivatization of a biomolecule by covalent coupling to the biomolecule in the presence of a directing methyltransferase. Further development of the method of targeted modification and derivatization are the method for targeted labeling a biomolecule and method for detecting unmethylated target sites in a biomolecule comprising modification of the biomolecule according to the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.