Patent · US Active

Thermostable luciferases and methods of production

US8822170B2 · kind B2 · utility

3Cited by

24References

19Claims

0Family size

Assignee

Promega Corporation · US

Inventors

Keith V. Wood · Verona, US
Monika G. Wood · Verona, US
Mary Hall · Waunakee, US

Key dates

Filing date	Aug 19, 2011
Grant date	Sep 2, 2014
Priority date	—
Expiry date	Aug 19, 2031

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S977/898
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.