Massively parallel 2-dimensional capillary electrophoresis

US8828209B2 · kind B2 · utility

Assignee

• The Research Foundation for The State University of New York · US

Inventors

• Vera Gorfinkel · Stony Brook, US
• Evgeni A. Kabotyanski · Stony Brook, US
• Boris Gorbovitski · Stony Brook, US

Key dates

• Filing date: Jun 22, 2006
• Grant date: Sep 9, 2014
• Expiry date: Nov 22, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/44782
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This invention discloses a highly efficient method, system and apparatus for nucleic acid analysis, including sequencing (both automated re-sequencing and de-novo sequencing). The system is capable of sequencing DNA sizes ranging from fragments to mammalian size genomes having mouse draft quality at a much reduced cost. The system comprises a massive parallel capillary electrophoretic separation using two-dimensional monolith multi-capillary arrays (2D-MMCA). Sequence identification can be performed using fluorescent or otherwise labeled dideoxynucleotide-terminated DNA extension product generated by gel matrix-, or beads-, or substrate tethered-, or otherwise immobilized colonies of single template molecules. Cost reduction is a significant advantage over currently known methods because of: (i) using massively parallel sub-nanoliter volume reactions; and (ii) employing 2D-MMCAs that increase the throughput of the CE separation and detection by at least two orders of magnitude compared to the commercial high-throughput DNA machines.