Patent · US Active

Method for the production of proteins and peptides

US8828670B2 · kind B2 · utility

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8Claims

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Assignee

RINA-NETWERK RNA TECHNOLOGIEN GMBH · DE

Inventors

Helmut Merk · Herrenberg, DE
Wolfgang Stiege · Berlin, DE
Christine Gless · Berlin, DE
Michael Gerrits · Berlin, DE

Key dates

Filing date	Jul 10, 2012
Grant date	Sep 9, 2014
Priority date	—
Expiry date	Jul 10, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2317/55
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a method for producing monomeric or dimeric proteins or peptides containing internal or external disulfide bonds, comprising the following steps: a) a cell-free lysate, obtainable from eukaryotic cells, is provided, which contains functional microsomal vesicles, b) a nucleic acid coding the protein or peptide and additionally containing a signal sequence is added to the lysate, c) the lysate with the nucleic acid is held for a given time at a temperature in the range from 20 to 35° C., proteins or peptides formed with the nucleic acid being translocated into the microsomal vesicles, d) the microsomal vesicles are then dissolved, and the proteins or peptides obtained thereby are optionally separated from the lysate.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.