Nucleic acids for detection and discrimination of genotypes of Chlamydophila psittaci

US8835117B2 · kind B2 · utility

Assignee

• The United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and Prevention · US

Inventors

• Stephanie Mitchell · Pasadena, US
• Jonas M. Winchell · Lilburn, US

Key dates

• Filing date: May 28, 2010
• Grant date: Sep 16, 2014
• Expiry date: Aug 18, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/689
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods of detecting Chlamydophila, including differentiating between species of Chlamydophila and/or strains of Chlamydophila psittaci are disclosed, for example to detect and genotype a Chlamydophila psittaci infection. A sample suspected of containing a nucleic acid of a Chlamydophila, is screened for the presence of that nucleic acid. The presence of the Chlamydophila nucleic acid indicates the presence of the Chlamydophila bacterium. Determining whether a Chlamydophila nucleic acid is present in a sample can be accomplished by detecting hybridization between a Chlamydophila specific primer, a Chlamydophila psittaci specific primer, and/or a Chlamydophila psittaci genotype-specific primer and the Chlamydophila nucleic acid containing sample. Thus, primers for the detection, species-specific and/or genotype-specific identification of Chlamydophila psittaci are disclosed. Kits that contain the disclosed primers also are disclosed.