Modifying single proteins (GPCR), ligands, and nanopore surfaces to create binding-induced molecular changes of protein-ligand complexes detected in nanochannel translocation

US8835362B2 · kind B2 · utility

Assignees

• International Business Machines Corporation · US
• The University of Melbourne · AU
• THE FLOREY INSTITUTE OF NEUROSCIENCE AND MENTAL HEALTH · AU

Inventors

• Matthew Downtown · Elmore, AU
• Natalie Gunn · Melbourne, AU
• Stefan Harrer · Hampton, AU
• Priscilla Rogers · Melbourne, AU
• John M. Wagner · Melbourne, AU
• Ross Alexander David Bathgate · Littlehampton, AU
• Daniel Scott · Elmore, AU
• Stan Skafidas · Elmore, AU

Key dates

• Filing date: Dec 26, 2012
• Grant date: Sep 16, 2014
• Expiry date: Dec 26, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A mechanism is provided for utilizing a nanodevice to distinguish molecules with different structure. The molecules translocate through or across a nanochannel filled with a electrolyte solution. An electrical signal through the nanochannel is measured for every translocation event. Inner surfaces of the nanochannel include a functional layer, which is a coating to functionalize the nanochannel, in which the functional layer is configured to interact with predetermined ones of the molecules during translocation events. It is determined that a combination of at least two different molecules is formed based on predetermined ones of the molecules interacting with the functional layer to change the electrical signal and/or change a translocation time for the translocation event.