Nanoparticle sensor for measuring protease activity and method for manufacturing the same
US8841085B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 27, 2009 |
| Grant date | Sep 23, 2014 |
| Priority date | — |
| Expiry date | Mar 28, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/953
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are a nanoparticle sensor for measuring protease activity, for protease imaging, and a method for preparing the same. More specifically, the present invention relates to a nanoparticle sensor for measuring protease activity in which a fluorophore- and a quencher-conjugated peptide substrate is conjugated to a biocompatible polymer nanoparticle. The peptide substrate is specifically lysed by a protease. The sensor according to the present invention is capable of inhibiting emission of fluorescence with high extinctive activity of the quencher on a fluorescent material. But strong fluorescence is specifically emitted only if the peptide substrate is lysed by a specific protease. Therefore, the sensor is especially useful as a method for screening a novel drug such as a protease overexpression inhibitor, and early diagnosis of incurable diseases and various diseases such as autoimmune diseases including cancer, osteoarthritis, rheumatoid arthritis and dementia.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.