Hybridoma cell line 10G4 and a monoclonal antibody against the total of aflatoxin B1, B2, G1 and G2

US8841419B2 · kind B2 · utility

Assignee

• OILCROPS RESEARCH INSTITUTE OF CHINESE ACADAMY OF AGRICULTURE SCIENCES · CN

Inventors

• Peiwu Li · Hubei, CN
• Xin Li · Foshan, CN
• Qi Zhang · Beijing, CN
• Xiaoxia Ding · Hubei, CN
• Wen Zhang · Hubei, CN
• Ran Li · Beijing, CN
• Zhaowei Zhang · Hubei, CN

Key dates

• Filing date: Jan 17, 2013
• Grant date: Sep 23, 2014
• Expiry date: Jan 17, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/577
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Hybridoma cell line 10G4 and monoclonal antibody against total aflatoxins produced by the hybridoma cell line 10G4. The hybridoma cell line 10G4 is used to produce the monoclonal antibody that binds specifically total aflatoxin B1, B2, G1 and G2. The titer of the mouse ascites antibody produced by the 10G4 treated mouse is determined through non-competitive enzyme-linked immunosorbent assay and the titer can reach up to 5.12×105. The monoclonal antibody against total aflatoxin B1, B2, G1 and G2 are used for better identification of aflatoxin B1, B2, G1 and G2 with good consistency. The 50% inhibitory concentrations (IC50) of the antibody against aflatoxin B1, B2, G1 and G2 are 2.09 ng/mL, 2.23 ng/mL, 2.19 ng/mL and 3.21 ng/mL respectively. The range of cross reaction rate for aflatoxin B1, B2, G1 and G2 is about 65.2%-100%. The antibody is used for quantitative measurement of total aflatoxin B1, B2, G1 and G2.