Method for creating perfusable microvessel systems
US8846307B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 14, 2009 |
| Grant date | Sep 30, 2014 |
| Priority date | — |
| Expiry date | Dec 5, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/50
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Creating a tissue structure in vitro includes juxtaposing mandrels on a culture/perfusion device frame where the mandrels are spaced apart substantially parallel to each other and connecting the mandrels to tubes including an upstream tubes and downstream tubes. The upstream tubes are connected with an upstream manifold and the downstream tubes are connected to a downstream manifold. The frame and the mandrels are sterilized, coated and seeded with cells that multiply and form circular layers around each of the mandrels until the circular layers merge into a tissue structure which is subjected to a growth medium. The mandrels are extracted and the tissue structure is perfused.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.