Nucleic acids encoding phosphate fluorescent indicators and methods of using the same

US8846365B2 · kind B2 · utility

Assignee

• Carnegie Institution of Washington · US

Inventors

• Wolf B. Frommer · Washington, US
• Hong Gu · Dongguan, CN
• Sylvie Lalonde · Stanford, US
• Arthur Grossman · Stanford, US

Key dates

• Filing date: Oct 14, 2005
• Grant date: Sep 30, 2014
• Expiry date: Jul 28, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/195
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Phosphate biosensors are disclosed, which comprise a phosphate binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon phosphate binding. Such biosensors are useful for real time monitoring of phosphate metabolism in living cells.