Aldehyde tags, uses thereof in site-specific protein modification

US8846866B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Isaac S. Carrico · Stony Brook, US
• Brian L. Carlson · Richmond, US
• Peng Wu · New Rochelle, US
• Carolyn R. Bertozzi · Berkeley, US

Key dates

• Filing date: Dec 7, 2012
• Grant date: Sep 30, 2014
• Expiry date: Dec 7, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/622
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.