Patent · US Active

Detection of multiple nucleic acid sequences in a reaction cartridge

US8852863B2 · kind B2 · utility

5Cited by

4References

7Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Thomas Rothmann · Langenfeld, DE
Holger Engel · Hilden, DE
Ralf Himmelreich · Langenfeld, DE
Andy Wende · Hilden, DE
Rainer Dahlke · Kaarst, DE

Key dates

Filing date	May 4, 2010
Grant date	Oct 7, 2014
Priority date	—
Expiry date	Mar 19, 2031

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY02A50/30
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.