Patent · US Active

Nucleic acid amplification procedure using RNA and DNA composite primers

US8852867B2 · kind B2 · utility

15Cited by
185References
10Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 9, 2011
Grant dateOct 7, 2014
Priority date—
Expiry dateMay 9, 2031

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6865
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA-containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.