Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same

US8853362B2 · kind B2 · utility

Assignee

• ESBATech, an Alcon Biomedical Research Unit LLC · CH

Inventors

• Kathrin Tissot · Schwanau, DE
• Stefan Ewert · Basel, CH
• Adrian Auf Der Maur · Dietikon, CH
• Alcide Barberis · Bioggio, CH
• Dominik Escher · Kreuzlingen, CH

Key dates

• Filing date: May 21, 2003
• Grant date: Oct 7, 2014
• Expiry date: Jun 12, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/94
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Compositions are provided, which can be used as frameworks for the creation of very stable and soluble single-chain Fv antibody fragments. These frameworks have been selected for intracellular performance and are thus ideally suited for the creation of scFv antibody fragments or scFv antibody libraries for applications where stability and solubility are limiting factors for the performance of antibody fragments, such as in the reducing environment of a cell. Such frameworks can also be used to identify highly conserved residues and consensus sequences which demonstrate enhanced solubility and stability.