Production of acetyl-coenzyme a derived isoprenoids
US8859261B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 24, 2013 |
| Grant date | Oct 14, 2014 |
| Priority date | — |
| Expiry date | Oct 24, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/08
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.