Cryopreservation of biological cells and tissues
US8859283B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 25, 2009 |
| Grant date | Oct 14, 2014 |
| Priority date | — |
| Expiry date | Aug 12, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N1/42
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The method involves placing an oocyte cell in a cell holder (1), securing the cell holder in a treatment station, applying a treatment solution to the cell by washing the cell with the solution, and rapidly cooling the cell holder and cell to a predetermined cryopreservation temperature for cryopreservation of the cell. The cell is cooled at a high rate sufficient to permit vitrification of the cell and any surrounding treatment solution to occur. The cell is then maintained at or below a predetermined storage temperature for storage. The method allows multiple cells to be treated simultaneously each secured within a respective cell holder.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.