Method for the selective enrichment and labeling of phosphorproteins
US8859734B2 · kind B2 · utility
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2References
28Claims
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Key dates
| Filing date | Sep 29, 2006 |
| Grant date | Oct 14, 2014 |
| Priority date | — |
| Expiry date | Oct 7, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K7/08
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The embodiments of the invention relate to a method for the introduction of a labeling structure such as a fluorescent molecules or a Raman tags to a compound. Imidazole functionalized resins or polymers are used to selectively immobilize phosphocompounds without protecting the carboxylic groups. Relying on the pKa difference between amines and hydrazides and carrying out the reaction in a slightly acidic buffer, all of the amines are protected by protonation while the hydrazides react with the phosphate imidazolide to form a phosphoramidate bond.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.