Methods and compositions for controlling assembly of viral proteins

US8865188B2 · kind B2 · utility

Assignee

• Biomed Realty, L.P. · US

Inventors

• Miguel de los Rios · Del Mar, US
• Stephanie de los Rios · San Diego, US
• Jacek Ostrowski · Lompoc, US
• Kenneth J. Oh · Thousand Oaks, US
• Ilan Zipkin · Belmont, US

Key dates

• Filing date: Sep 10, 2012
• Grant date: Oct 21, 2014
• Expiry date: Sep 10, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2730/10122
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided herein are methods and compositions for controlling assembly of modified viral core proteins, for example, into a viral capsid or a nanocage. In some embodiments, the disclosed modified viral core proteins comprise at least one mutation or modification that can substantially prevent assembly of the viral core proteins until assembly is desired. In some embodiments, assembly of the viral core proteins may be triggered, for example, by contacting the viral core proteins with a reducing agent and/or by reducing the concentration of a denaturant. The viral core proteins may self-assemble to form a viral capsid or nanocage.