Biomatrix composition and methods of biomatrix seeding

US8865199B2 · kind B2 · utility

Assignees

• INGENERON, INC. · US
• The Board of Regents of the University of Texas System · US

Inventors

• Michael E. Coleman · Pompano Beach, US
• Eckhard Alt · Ottobrunn, DE
• Ron Stubbers · Houston, US

Key dates

• Filing date: Nov 17, 2009
• Grant date: Oct 21, 2014
• Expiry date: Feb 19, 2032

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61L2430/40
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Apparatus and methods are described for generating autologous tissue grafts, the apparatus including a point of care SVF isolation unit that includes a tissue digestion chamber in fluid communication with a lipid separating chamber, whereby SVF cells are isolated without centrifugation; and a cell seeding chamber in fluid communication with the SVF isolation unit, said cell seeding chamber adapted to support a cell scaffold. Methods and materials for cell seeding, including through the provision of micro rough scaffold surfaces, are also provided.