Assays for nuclear hormone receptor binding
US8865421B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | Jun 23, 2009 |
| Grant date | Oct 21, 2014 |
| Priority date | — |
| Expiry date | Sep 6, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and genetic constructs are provided for detecting the binding of nuclear hormone receptors to a coactivator/corepressor. The methods employ enzyme fragment complementation using fragments of β-galactosidase as the detection system. Cells are transformed to express the large fragment of β-galactosidase fused to a member of the complex with NHR for initiation of transcription and have it localized in the nucleus and to express the small fragment of β-galactosidase fused to the nuclear hormone receptor for binding to the member upon stimulation with a ligand.
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