Microfluidic cell culture device

US8865464B2 · kind B2 · utility

Assignee

• THE REGENTS OF THE UNIVERSITY OF MICHIGAN · US

Inventors

• Shuichi Takayama · Ann Arbor, US
• Lourdes Marcella Cabrera · Ann Arbor, US
• Yun Seok Heo · Daegu, KR
• Gary D. Smith · Ann Arbor, US

Key dates

• Filing date: Aug 11, 2010
• Grant date: Oct 21, 2014
• Expiry date: Aug 11, 2030

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L3/5085
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Microfluidic devices for cell culturing and methods for using the same are disclosed. One device includes a substrate and membrane. The substrate includes a reservoir in fluid communication with a passage. A bio-compatible fluid may be added to the reservoir and passage. The reservoir is configured to receive and retain at least a portion of a cell mass. The membrane acts as a barrier to evaporation of the bio-compatible fluid from the passage. A cover fluid may be added to cover the bio-compatible fluid to prevent evaporation of the bio-compatible fluid.