Methods and compositions for identifying agents that inhibit an NS4B-mediated neoplastic cellular phenotype of HCV infected cells

US8871435B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Jeffrey S. Glenn · Palo Alto, US
• Shirit Einav · Stanford, US

Key dates

• Filing date: Jun 20, 2008
• Grant date: Oct 28, 2014
• Expiry date: Mar 18, 2031

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K49/0008
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods and compositions for identifying agents that inhibit a neoplastic cellular phenotype mediated by the NS4B protein nucleotide binding motif (NBM) of hepatitis C virus (HCV) are provided. In general, the methods involve contacting a candidate agent with a mammalian cell expressing an NS4B NBM polypeptide of an HCV virus, wherein expression of the NS4B NBM polypeptide in the absence of candidate agent promotes a neoplastic cellular phenotype, and detecting the presence or absence of an effect of the candidate agent on NS4B-mediated promotion of a neoplastic cellular phenotype. The provided methods and compositions find use in a variety of therapeutic and screening applications.