Construction of protein-responsive shRNA/RNAi control system using RNP motif
US8871437B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 9, 2009 |
| Grant date | Oct 28, 2014 |
| Priority date | — |
| Expiry date | May 30, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/50
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An object of the present invention is to provide an RNAi control system using an RNA-protein interaction motif. The present invention provides an shRNA comprising: a guide strand having a sequence complementary to a target sequence; a passenger strand which forms a duplex with the guide strand; and a linker strand which links the guide strand and the passenger strand, wherein the linker strand comprises an RNP-derived protein-binding motif sequence. The present invention also provides an RNAi control system comprising: the shRNA; and an RNP-derived protein which specifically binds to a protein-binding motif sequence in the shRNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.