Microfluidic device screening method
US8871441B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 14, 2009 |
| Grant date | Oct 28, 2014 |
| Priority date | — |
| Expiry date | Aug 14, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/99
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods of screening a microbial host cell for a property of interest in a microfluidic device, the method comprising the steps of: a) transforming a d-alanine racemase-deficient microbial host cell with a polynucleotide construct comprising: i) one or more polynucleotide region providing the property of interest when present in the host cell, and ii) at least one polynucleotide region complementing the d-alanine racemase deficiency when present in the host cell; and b) screening the transformed host cell for the property of interest in the microfluidic device in the absence of externally provided d-alanine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.