In vitro evolution in microfluidic systems
US8871444B2 · kind B2 · utility
287Cited by
434References
9Claims
0Family size
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Key dates
| Filing date | Dec 4, 2012 |
| Grant date | Oct 28, 2014 |
| Priority date | — |
| Expiry date | Dec 4, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/149
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalizing genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.