Method for identifying bacteria from the Bacillus cereus group
US8871465B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 16, 2010 |
| Grant date | Oct 28, 2014 |
| Priority date | — |
| Expiry date | Sep 16, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/44
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for identifying bacteria of the Bacillus cereus group, comprising the following steps: (a) providing a sample that may contain bacteria of the Bacillus cereus group, a reaction medium comprising at least one fluorescent phosphatidylcholine phospholipase C (PC-PLC) substrate and an inhibitor of Gram-negative bacteria; (b) inoculating the reaction medium with the sample; (c) incubating the inoculated reaction medium; and (d) identifying the bacteria of the Bacillus cereus group by detecting the PC-PLC substrate hydrolysis reaction, in which the pH of the reaction medium and the time necessary for detecting the PC-PLC substrate hydrolysis reaction are adapted such that said hydrolysis reaction by bacteria of the Bacillus cereus group is detected before hydrolysis of the PC-PLC substrate by any Gram-positive bacteria other than those belonging to the Bacillus cereus group, that may be present in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.