Methods of identifying a pair of binding partners
US8871686B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 7, 2010 |
| Grant date | Oct 28, 2014 |
| Priority date | — |
| Expiry date | Jul 7, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to methods of identifying a binding partner of a target molecule within a plurality of analyte molecules, including a plurality of peptides and/or proteins. The target molecule is physically combined with a target labeling nucleic acid molecule. Each member of the plurality of analyte molecules is physically linked to an analyte labeling nucleic acid molecule, each analyte labeling nucleic acid molecule comprising a selected nucleotide sequence. This specific nucleotide sequence may include a sequence encoding a peptide/protein combined therewith. The target molecule is contacted with the analyte molecules and a complex between the target molecule and an analyte molecule forms. The mixture is subdivided into compartments. The target labeling nucleic acid molecule and the analyte labeling nucleic acid molecule are linked and the plurality of compartments allowed to disintegrate. The linked nucleic acid molecule is retrieved and the sequence determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.