Patent · US Active

Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule

US8877504B2 · kind B2 · utility

4Cited by

17References

6Claims

0Family size

Assignee

UNIVERSITÉ LIBRE DE BRUXELLES · BE

Inventors

Philippe Gabant · Brussels, BE
Laurence Van Melderen · Waterloo, BE
Cedric Szpirer · Fleurus, BE

Key dates

Filing date	Jun 17, 2013
Grant date	Nov 4, 2014
Priority date	—
Expiry date	Jun 17, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/66
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention is related to a method for the selection of recombinant clones having integrated a gene of interest and a nucleotide sequence encoding a functional antidote protein to a toxic molecule, wherein said recombinant clones are the ones which survive following their integration into a host cell comprising in its genome a nucleotide sequence encoding said toxic molecule. The present invention is also related to a nucleic acid construct, a vector comprising said nucleic acid construct, a host cell and a cloning and/or sequencing kit for performing said method.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.