Microscopy method for identifying biological target objects

US8879792B2 · kind B2 · utility

Assignee

• Sensovation AG · DE

Inventors

• Paul Hing · Sigmaringen, DE
• Stefan Bickert · Überlingen, DE

Key dates

• Filing date: Nov 16, 2012
• Grant date: Nov 4, 2014
• Expiry date: Jun 19, 2033

Classification

• Technology area (CPC H): Electricity
• CPC primary: H04N7/18
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a microscopy method for identifying target objects (32) having a predetermined optical property in material (6) to be analyzed.According to the invention, in a first step an overview field of view (36) of a microscope optical system (14) is directed to an overview region of a sample carrier (4) containing the material (6) to be analyzed, the material (6) to be analyzed is illuminated by an illumination unit (16), which irradiates the sample carrier (4) from outside a field of view tube (48), and is recorded by a camera (8), the material (6) to be analyzed is optically analyzed for the optical property such that even a single target object (32) having the predetermined optical property is identified as such in the material (6) to be analyzed, in a subsequent second step a target field of view (52) of the microscope optical system (14) is aligned with a target region around the target object (32) using the known position of the target object (32), and the identified target object (32) is analyzed in a differentiated manner for various additional optical properties.