Switchable nucleic acid aptamer probe and uses thereof in tumor living cell detection and in vivo detection
US8883994B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 22, 2011 |
| Grant date | Nov 11, 2014 |
| Priority date | — |
| Expiry date | Mar 22, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6432
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A switch mode nucleic acid aptamer probe includes a probe main body, a fluorescence generating unit and a fluorescence quenching unit which are respectively connected to two ends of the probe main body. The probe main body includes a nucleic acid aptamer fragment with a function of specifically recognizing target tumor cell and a nucleic acid fragment linked to the nucleic acid aptamer fragment by a connection fragment with a length of 7˜15 nm so as to form a hairpin structure. The ability of competitive hybridization of the nucleic acid fragment with the nucleic acid aptamer fragment is weaker than that of the target tumor cell. The use of the probe of the invention can be at least one of specific detection of tumor living cell in buffer solution, effective detection of tumor living cell in serum, and real-time fluorescence imaging and intravital detection of tumor in living body.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.