Conversion of alpha-hydroxyalkylated residues in biomolecules using methyltransferases
US8889352B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 1, 2010 |
| Grant date | Nov 18, 2014 |
| Priority date | — |
| Expiry date | Sep 5, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/91011
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to targeted conversion of alpha-hydroxyalkylated residues in biomolecules in the presence of a directing methyltransferase, namely to targeted removal of the alpha-hydroxyalkyl moieties to give unmodified residues, or targeted derivatization of the alpha-hydroxyalkyl groups by covalent coupling of non-cofactor compounds represented by formula HQ-LX1 wherein X represents a functional group or a reporter group attached via a linker moiety L, and QH is selected from HS—, HSe—, HO—H2N—, HN3 or HCN in the presence of a directing methyltransferase. Further development of the method of targeted conversion comprises methods for targeted labeling a biomolecule and method for detecting hydroxymethylated target sites in a biomolecule according to the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.