Cell classification system
US8889362B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 30, 2007 |
| Grant date | Nov 18, 2014 |
| Priority date | — |
| Expiry date | Apr 4, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/70514
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention involves the reaction of a CD4 immuno-conjugate with a sample of patient whole blood. The CD4 immuno-conjugate consists of one or more antibodies with specificity for the CD4 surface receptor coupled to a signal moiety, or “label”, that is detectable by a flow cytometer. Such labels may generate a signal by such means as fluorescence properties, light scatter properties, electronic properties, or magnetic properties. The CD4 immuno-conjugate binds to both the CD4 positive lymphocytes (Helper T cells) and all monocytes. Differential detection means are employed to count immuno-conjugate labeled Helper T cells. The present invention distinguishes itself by simultaneously measuring the signal level from monocytes as a means to verify sufficient activity of the anti-CD4 antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.