Methods and devices for micro-isolation, extraction, and/or analysis of microscale components

US8889416B2 · kind B2 · utility

Assignees

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US
• University of Southern California · US

Inventors

• Emil P. Kartalov · Pasadena, US
• Darryl Shibata · Los Angeles, US
• Clive R. Taylor · Hertford, GB
• Lawrence A. Wade · Fremont, US

Key dates

• Filing date: Jan 20, 2011
• Grant date: Nov 18, 2014
• Expiry date: Nov 7, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54366
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the apparatuses described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein.