3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
US8889860B2 · kind B2 · utility
6Cited by
49References
35Claims
0Family size
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Key dates
| Filing date | Sep 13, 2012 |
| Grant date | Nov 18, 2014 |
| Priority date | — |
| Expiry date | Oct 27, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention relates generally to 3′-OH unblocked, labeled and unlabeled nucleotides and nucleosides with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds, which may be represented by the following formulas:wherein the variables are defined herein, may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.