Single chain trimers and uses therefor
US8895020B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 4, 2006 |
| Grant date | Nov 25, 2014 |
| Priority date | — |
| Expiry date | Nov 3, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Single chain trimer (SCT) molecules are disclosed, comprising an MHC antigen peptide sequence, a β2-microglobulin sequence and a full-length MHC class I heavy chain sequence, joined by linker sequences. Further described are nucleic acids encoding single chain trimers. Methods for expansion of antigen-specific T cell populations using single chain trimer molecules are also disclosed. In some configurations, these methods comprise co-culturing, in a first stage, CD8+ T cells from a donor with antigen presenting cells comprising an MHC antigen peptide, and co-culturing, in a second stage, the CD8+ T cells with cells comprising an SCT which has an MHC antigen peptide sequence identical to the sequence of the antigen peptide in the first stage. The methods can provide 10,000-100,000 fold expansion of antigen-specific CD8+ T cells within about 28 days after establishing culture, and can yield over 1 billion antigen-specific CD8+ T cells expanded from an individual donor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.