L-rhamnose-inducible expression systems

US8895310B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Maria Keβeler · Mannheim, DE
• Thomas Zelinski · Menasha, US
• Bernhard Hauer · Neheim, DE

Key dates

• Filing date: Nov 27, 2003
• Grant date: Nov 25, 2014
• Expiry date: Jul 21, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells, where at least one DNA construct which is capable of episomal replication in a host cell and which comprises a nucleic acid sequence to be expressed under the transcriptional control of an L-rhamnose-inducible promoter, where the promoter is heterologous with regard to the nucleic acid sequence, is introduced into the host cell and the expression of he nucleic acid sequence is induced by addition of L-rhamnose, wherein the prokaryotic host cell is at least deficient with regard to an L-rhamnose isomerase.