Isolation and purification of antibodies using protein A affinity chromatography
US8895709B2 · kind B2 · utility
53Cited by
41References
18Claims
0Family size
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Inventors
Key dates
| Filing date | Oct 20, 2009 |
| Grant date | Nov 25, 2014 |
| Priority date | — |
| Expiry date | Dec 18, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/824
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.