Patent · US Active

Microfluidic chamber device and fabrication

US8900529B2 · kind B2 · utility

24Cited by
11References
10Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 27, 2012
Grant dateDec 2, 2014
Priority date
Expiry dateDec 3, 2032

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T156/108
  • WIPO fieldSurface technology, coating
  • WIPO sectorChemistry

Abstract

A microfluidic flow cell subassembly, which may be assembled into a flow cell having fluidic connections outside of the main substrate, is described for encapsulating a sample to allow for subsequent controlled delivery of reagents to the sample, such as multiplexed in situ biomarker staining and analysis. Methods for fabricating the subassembly and assembled flow cell are also provided. The method includes the steps of adhering a gasket layer to a substrate layer, wherein the gasket layer may contain enclosed features and adhering an adherent layer to the gasket layer. The subassembly may be sealed against a solid support to form a flow cell.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.