Fibrinogen assay
US8900822B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2011 |
| Grant date | Dec 2, 2014 |
| Priority date | — |
| Expiry date | Jun 17, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/447
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to a method of detecting intact fibrinogen, comprising the steps of: a) providing a sample containing at least some fibrinogen optionally converted at least in part to fibrin, and optionally containing thrombin; b) solubilizing the sample in a solubilizing solution that inhibits thrombin activity; c) after optional SDS-PAGE transferring/applying a portion of said sample to a protein-binding membrane; d) reacting the fibrinogen with a primary monoclonal antibody capable of binding to fibrinopeptide A moiety; and e) detecting the quantity of intact fibrinogen in the sample by quantifying the amount of the bound primary monoclonal antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.