FRT recombination sites and methods of use

US8900869B2 · kind B2 · utility

Assignee

• PIONEER HI-BRED INTERNATIONAL, INC. · US

Inventors

• Yumin Tao · Ames, US
• Dennis L. Bidney · Urbandale, US
• William J. Gordon-Kamm · Urbandale, US
• Leszek A. Lyznik · Johnston, US

Key dates

• Filing date: Oct 25, 2012
• Grant date: Dec 2, 2014
• Expiry date: Oct 25, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/08
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.