Analytical method of post-translational modifications in hemoglobin
US8906605B2 · kind B2 · utility
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Key dates
| Filing date | Jun 27, 2013 |
| Grant date | Dec 9, 2014 |
| Priority date | — |
| Expiry date | Jun 27, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6848
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An analytical method of post-translational modifications in hemoglobin is disclosed. The analytical method comprises the steps of providing a blood comprising the hemoglobin with post-translational modification of nitration, nitrosylation, or oxidation; performing an extraction process to the blood by an organic solvent; quantifying the hemoglobin by a fluorescent spectrometry; hydrolyzing the hemoglobin into a plurality of peptides by an enzyme; and using a nanoflow liquid chromatography-nanospray ionization tandem mass spectrometry to characterize and quantify the post-translational modifications of the hemoglobin.
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